Construct: shRNA TRCN0000158346

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018538.2-665s1c1
DNA Barcode:
CTTCTCATCATGGTGTGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERMAP (114625)
Gene Description:
erythroblast membrane associated protein (Scianna blood group)
Transcript:
RefSeq NM_018538.2 (NON-CURRENT)
Match location:
Position 665 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158346)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTCATCATGGTGTGCCTT
Hairpin Sequence:
5'-CCGG-CTTCTCATCATGGTGTGCCTT-CTCGAG-AAGGCACACCATGATGAGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTCATCATGGTGTGCCTTCTCGAGAAGGCACACCATGATGAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTCATCATGGTGTGCCTTCTCGAGAAGGCACACCATGATGAGAAG-3'

Other clones with same target sequence:

(none)