Construct: shRNA TRCN0000158414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053016.1-150s1c1
DNA Barcode:
CAGATACTTCTGCTGCAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PALM2 (114299)
Gene Description:
paralemmin 2
Transcript:
RefSeq NM_053016.1 (NON-CURRENT)
Match location:
Position 150 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATACTTCTGCTGCAGCAT
Hairpin Sequence:
5'-CCGG-CAGATACTTCTGCTGCAGCAT-CTCGAG-ATGCTGCAGCAGAAGTATCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATACTTCTGCTGCAGCATCTCGAGATGCTGCAGCAGAAGTATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATACTTCTGCTGCAGCATCTCGAGATGCTGCAGCAGAAGTATCTG-3'

Other clones with same target sequence:

(none)