Construct: shRNA TRCN0000158488

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024457.1-1477s1c1
DNA Barcode:
CCACTTACAATTAAAGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGPD1 (400966)
Gene Description:
RANBP2 like and GRIP domain containing 1
Transcript:
RefSeq NM_001024457.1 (NON-CURRENT)
Match location:
Position 1477 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158488)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTACAATTAAAGGAGAA
Hairpin Sequence:
5'-CCGG-CCACTTACAATTAAAGGAGAA-CTCGAG-TTCTCCTTTAATTGTAAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTACAATTAAAGGAGAACTCGAGTTCTCCTTTAATTGTAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTACAATTAAAGGAGAACTCGAGTTCTCCTTTAATTGTAAGTGG-3'

Other clones with same target sequence:

(none)