Construct: shRNA TRCN0000158561

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020425.3-79s1c1
DNA Barcode:
CCAAAGAGAAAGAGTTTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMIM8 (57150)
Gene Description:
small integral membrane protein 8
Transcript:
RefSeq NM_020425.3 (NON-CURRENT)
Match location:
Position 79 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158561)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGAGAAAGAGTTTCAAA
Hairpin Sequence:
5'-CCGG-CCAAAGAGAAAGAGTTTCAAA-CTCGAG-TTTGAAACTCTTTCTCTTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGAGAAAGAGTTTCAAACTCGAGTTTGAAACTCTTTCTCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGAGAAAGAGTTTCAAACTCGAGTTTGAAACTCTTTCTCTTTGG-3'

Other clones with same target sequence:

(none)