Construct: shRNA TRCN0000158640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003134.2-585s1c1
DNA Barcode:
CACATAAGATTAGGGTCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRP14 (6727)
Gene Description:
signal recognition particle 14
Transcript:
RefSeq NM_003134.2 (NON-CURRENT)
Match location:
Position 585 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACATAAGATTAGGGTCCTTT
Hairpin Sequence:
5'-CCGG-CACATAAGATTAGGGTCCTTT-CTCGAG-AAAGGACCCTAATCTTATGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACATAAGATTAGGGTCCTTTCTCGAGAAAGGACCCTAATCTTATGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACATAAGATTAGGGTCCTTTCTCGAGAAAGGACCCTAATCTTATGTG-3'

Other clones with same target sequence:

  1. TRCN0000343412