Construct: shRNA TRCN0000158685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016396.1-2404s1c1
DNA Barcode:
GCACACAGATTTAATGGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTDSPL2 (51496)
Gene Description:
CTD small phosphatase like 2
Transcript:
RefSeq NM_016396.1 (NON-CURRENT)
Match location:
Position 2404 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACAGATTTAATGGATAA
Hairpin Sequence:
5'-CCGG-GCACACAGATTTAATGGATAA-CTCGAG-TTATCCATTAAATCTGTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACAGATTTAATGGATAACTCGAGTTATCCATTAAATCTGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACAGATTTAATGGATAACTCGAGTTATCCATTAAATCTGTGTGC-3'

Other clones with same target sequence:

(none)