Construct: shRNA TRCN0000158783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176815.2-709s1c1
DNA Barcode:
GAAGTATGTGAGAAGGATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHFR2 (200895)
Gene Description:
dihydrofolate reductase 2
Transcript:
RefSeq NM_176815.2 (NON-CURRENT)
Match location:
Position 709 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTATGTGAGAAGGATGAT
Hairpin Sequence:
5'-CCGG-GAAGTATGTGAGAAGGATGAT-CTCGAG-ATCATCCTTCTCACATACTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTATGTGAGAAGGATGATCTCGAGATCATCCTTCTCACATACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTATGTGAGAAGGATGATCTCGAGATCATCCTTCTCACATACTTC-3'

Other clones with same target sequence:

(none)