Construct: shRNA TRCN0000158865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_147194.1-1902s1c1
DNA Barcode:
GCTTTGAATATCTGACTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM185A (222234)
Gene Description:
family with sequence similarity 185 member A
Transcript:
RefSeq NM_147194.1 (NON-CURRENT)
Match location:
Position 1902 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGAATATCTGACTTCTT
Hairpin Sequence:
5'-CCGG-GCTTTGAATATCTGACTTCTT-CTCGAG-AAGAAGTCAGATATTCAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGAATATCTGACTTCTTCTCGAGAAGAAGTCAGATATTCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGAATATCTGACTTCTTCTCGAGAAGAAGTCAGATATTCAAAGC-3'

Other clones with same target sequence:

(none)