Construct: shRNA TRCN0000158866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152383.2-1175s1c1
DNA Barcode:
GAAGGAATACTAACAGAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DIS3L2 (129563)
Gene Description:
DIS3 like 3'-5' exoribonuclease 2
Transcript:
RefSeq NM_152383.2 (NON-CURRENT)
Match location:
Position 1175 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGAATACTAACAGAGTAT
Hairpin Sequence:
5'-CCGG-GAAGGAATACTAACAGAGTAT-CTCGAG-ATACTCTGTTAGTATTCCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGAATACTAACAGAGTATCTCGAGATACTCTGTTAGTATTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGAATACTAACAGAGTATCTCGAGATACTCTGTTAGTATTCCTTC-3'

Other clones with same target sequence:

(none)