Construct: shRNA TRCN0000158881

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015024.2-347s1c1
DNA Barcode:
GCGAATAGATATTCGGAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPO7 (23039)
Gene Description:
exportin 7
Transcript:
RefSeq NM_015024.2 (NON-CURRENT)
Match location:
Position 347 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158881)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAATAGATATTCGGAACTA
Hairpin Sequence:
5'-CCGG-GCGAATAGATATTCGGAACTA-CTCGAG-TAGTTCCGAATATCTATTCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAATAGATATTCGGAACTACTCGAGTAGTTCCGAATATCTATTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAATAGATATTCGGAACTACTCGAGTAGTTCCGAATATCTATTCGC-3'

Other clones with same target sequence:

  1. TRCN0000338891