Construct: shRNA TRCN0000158916

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022720.4-1832s1c1
DNA Barcode:
CCAATCAGAAGCTCATTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGCR8 (54487)
Gene Description:
DGCR8 microprocessor complex subunit
Transcript:
RefSeq NM_022720.4 (NON-CURRENT)
Match location:
Position 1832 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158916)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATCAGAAGCTCATTACTT
Hairpin Sequence:
5'-CCGG-CCAATCAGAAGCTCATTACTT-CTCGAG-AAGTAATGAGCTTCTGATTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATCAGAAGCTCATTACTTCTCGAGAAGTAATGAGCTTCTGATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATCAGAAGCTCATTACTTCTCGAGAAGTAATGAGCTTCTGATTGG-3'

Other clones with same target sequence:

(none)