Construct: shRNA TRCN0000158937

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020991.3-389s1c1
DNA Barcode:
GAAACGCAACAGAAATCCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSH2 (1443)
Gene Description:
chorionic somatomammotropin hormone 2
Transcript:
RefSeq NM_020991.3 (CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158937)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACGCAACAGAAATCCAAT
Hairpin Sequence:
5'-CCGG-GAAACGCAACAGAAATCCAAT-CTCGAG-ATTGGATTTCTGTTGCGTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACGCAACAGAAATCCAATCTCGAGATTGGATTTCTGTTGCGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACGCAACAGAAATCCAATCTCGAGATTGGATTTCTGTTGCGTTTC-3'

Other clones with same target sequence:

  1. TRCN0000083817