Construct: shRNA TRCN0000158950

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002725.3-3868s1c1
DNA Barcode:
GCTGGTTTCTTTCAAGTTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRELP (5549)
Gene Description:
proline and arginine rich end leucine rich repeat protein
Transcript:
RefSeq NM_002725.3 (NON-CURRENT)
Match location:
Position 3868 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158950)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTTTCTTTCAAGTTTAA
Hairpin Sequence:
5'-CCGG-GCTGGTTTCTTTCAAGTTTAA-CTCGAG-TTAAACTTGAAAGAAACCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTTTCTTTCAAGTTTAACTCGAGTTAAACTTGAAAGAAACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTTTCTTTCAAGTTTAACTCGAGTTAAACTTGAAAGAAACCAGC-3'

Other clones with same target sequence:

(none)