Construct: shRNA TRCN0000158978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152745.1-2040s1c1
DNA Barcode:
GCTTTCATTGTCTGACTCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NXPH1 (30010)
Gene Description:
neurexophilin 1
Transcript:
RefSeq NM_152745.1 (NON-CURRENT)
Match location:
Position 2040 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCATTGTCTGACTCATA
Hairpin Sequence:
5'-CCGG-GCTTTCATTGTCTGACTCATA-CTCGAG-TATGAGTCAGACAATGAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCATTGTCTGACTCATACTCGAGTATGAGTCAGACAATGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCATTGTCTGACTCATACTCGAGTATGAGTCAGACAATGAAAGC-3'

Other clones with same target sequence:

(none)