Construct: shRNA TRCN0000158995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017912.2-3411s1c1
DNA Barcode:
GCATAAGGTATCAAACCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HERC6 (55008)
Gene Description:
HECT and RLD domain containing E3 ubiquitin protein ligase family member 6
Transcript:
RefSeq NM_017912.2 (NON-CURRENT)
Match location:
Position 3411 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATAAGGTATCAAACCACAT
Hairpin Sequence:
5'-CCGG-GCATAAGGTATCAAACCACAT-CTCGAG-ATGTGGTTTGATACCTTATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATAAGGTATCAAACCACATCTCGAGATGTGGTTTGATACCTTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATAAGGTATCAAACCACATCTCGAGATGTGGTTTGATACCTTATGC-3'

Other clones with same target sequence:

(none)