Construct: shRNA TRCN0000159110

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005821.2-615s1c1
DNA Barcode:
GATTCCTAAATCCCTTGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NBR2 (10230)
Gene Description:
neighbor of BRCA1 lncRNA 2
Transcript:
RefSeq NM_005821.2 (NON-CURRENT)
Match location:
Position 615 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159110)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCCTAAATCCCTTGGAAT
Hairpin Sequence:
5'-CCGG-GATTCCTAAATCCCTTGGAAT-CTCGAG-ATTCCAAGGGATTTAGGAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCCTAAATCCCTTGGAATCTCGAGATTCCAAGGGATTTAGGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCCTAAATCCCTTGGAATCTCGAGATTCCAAGGGATTTAGGAATC-3'

Other clones with same target sequence:

(none)