Construct: shRNA TRCN0000159129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016396.1-2595s1c1
DNA Barcode:
GCTCTCAGTTACAATCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTDSPL2 (51496)
Gene Description:
CTD small phosphatase like 2
Transcript:
RefSeq NM_016396.1 (NON-CURRENT)
Match location:
Position 2595 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCAGTTACAATCAATTT
Hairpin Sequence:
5'-CCGG-GCTCTCAGTTACAATCAATTT-CTCGAG-AAATTGATTGTAACTGAGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCAGTTACAATCAATTTCTCGAGAAATTGATTGTAACTGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCAGTTACAATCAATTTCTCGAGAAATTGATTGTAACTGAGAGC-3'

Other clones with same target sequence:

(none)