Construct: shRNA TRCN0000159164

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018191.2-1448s1c1
DNA Barcode:
GAAGACATGAAGGAAGTGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RCBTB1 (55213)
Gene Description:
RCC1 and BTB domain containing protein 1
Transcript:
RefSeq NM_018191.2 (NON-CURRENT)
Match location:
Position 1448 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159164)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGACATGAAGGAAGTGATA
Hairpin Sequence:
5'-CCGG-GAAGACATGAAGGAAGTGATA-CTCGAG-TATCACTTCCTTCATGTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGACATGAAGGAAGTGATACTCGAGTATCACTTCCTTCATGTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGACATGAAGGAAGTGATACTCGAGTATCACTTCCTTCATGTCTTC-3'

Other clones with same target sequence:

(none)