Construct: shRNA TRCN0000159240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001010916.1-549s1c1
DNA Barcode:
GAATTGATAGTGAGGGTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHCHD2P9 (645345)
Gene Description:
coiled-coil-helix-coiled-coil-helix domain containing 2 pseudogene 9
Transcript:
RefSeq NM_001010916.1 (NON-CURRENT)
Match location:
Position 549 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATTGATAGTGAGGGTATAA
Hairpin Sequence:
5'-CCGG-GAATTGATAGTGAGGGTATAA-CTCGAG-TTATACCCTCACTATCAATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATTGATAGTGAGGGTATAACTCGAGTTATACCCTCACTATCAATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATTGATAGTGAGGGTATAACTCGAGTTATACCCTCACTATCAATTC-3'

Other clones with same target sequence:

(none)