Construct: shRNA TRCN0000159311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017436.1-273s1c1
DNA Barcode:
GTAGATCCTGAAACTGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CT45A4 (441520)
Gene Description:
cancer/testis antigen family 45, member A4
Transcript:
RefSeq NM_001017436.1 (NON-CURRENT)
Match location:
Position 273 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGATCCTGAAACTGTGTTT
Hairpin Sequence:
5'-CCGG-GTAGATCCTGAAACTGTGTTT-CTCGAG-AAACACAGTTTCAGGATCTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGATCCTGAAACTGTGTTTCTCGAGAAACACAGTTTCAGGATCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGATCCTGAAACTGTGTTTCTCGAGAAACACAGTTTCAGGATCTAC-3'

Other clones with same target sequence:

  1. TRCN0000150185