Construct: shRNA TRCN0000159340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020178.2-1616s1c1
DNA Barcode:
GAAGCTTCAGTATAGAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CA10 (56934)
Gene Description:
carbonic anhydrase 10
Transcript:
RefSeq NM_020178.2 (NON-CURRENT)
Match location:
Position 1616 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCTTCAGTATAGAGTAAA
Hairpin Sequence:
5'-CCGG-GAAGCTTCAGTATAGAGTAAA-CTCGAG-TTTACTCTATACTGAAGCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCTTCAGTATAGAGTAAACTCGAGTTTACTCTATACTGAAGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCTTCAGTATAGAGTAAACTCGAGTTTACTCTATACTGAAGCTTC-3'

Other clones with same target sequence:

(none)