Construct: shRNA TRCN0000159372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018243.2-741s1c1
DNA Barcode:
GCACAAATTCAAGAGTAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEPTIN11 (55752)
Gene Description:
septin 11
Transcript:
RefSeq NM_018243.2 (NON-CURRENT)
Match location:
Position 741 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAATTCAAGAGTAAGAT
Hairpin Sequence:
5'-CCGG-GCACAAATTCAAGAGTAAGAT-CTCGAG-ATCTTACTCTTGAATTTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAATTCAAGAGTAAGATCTCGAGATCTTACTCTTGAATTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAATTCAAGAGTAAGATCTCGAGATCTTACTCTTGAATTTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000281001
  2. TRCN0000112986
  3. TRCN0000326491