Construct: shRNA TRCN0000159393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003716.2-4715s1c1
DNA Barcode:
GCTTCATTCATGTACTATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CADPS (8618)
Gene Description:
calcium dependent secretion activator
Transcript:
RefSeq NM_003716.2 (NON-CURRENT)
Match location:
Position 4715 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCATTCATGTACTATGTA
Hairpin Sequence:
5'-CCGG-GCTTCATTCATGTACTATGTA-CTCGAG-TACATAGTACATGAATGAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCATTCATGTACTATGTACTCGAGTACATAGTACATGAATGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCATTCATGTACTATGTACTCGAGTACATAGTACATGAATGAAGC-3'

Other clones with same target sequence:

(none)