Construct: shRNA TRCN0000159423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016522.2-1206s1c1
DNA Barcode:
GTGAAGACGAATACTTGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTM (50863)
Gene Description:
neurotrimin
Transcript:
RefSeq NM_016522.2 (CURRENT)
Match location:
Position 1206 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAAGACGAATACTTGGAAA
Hairpin Sequence:
5'-CCGG-GTGAAGACGAATACTTGGAAA-CTCGAG-TTTCCAAGTATTCGTCTTCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAAGACGAATACTTGGAAACTCGAGTTTCCAAGTATTCGTCTTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAAGACGAATACTTGGAAACTCGAGTTTCCAAGTATTCGTCTTCAC-3'

Other clones with same target sequence:

(none)