Construct: shRNA TRCN0000159437

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153010.3-652s1c1
DNA Barcode:
GCACCTACTATGTGCATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AQP4-AS1 (147429)
Gene Description:
AQP4 antisense RNA 1
Transcript:
RefSeq NM_153010.3 (NON-CURRENT)
Match location:
Position 652 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159437)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTACTATGTGCATTATT
Hairpin Sequence:
5'-CCGG-GCACCTACTATGTGCATTATT-CTCGAG-AATAATGCACATAGTAGGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTACTATGTGCATTATTCTCGAGAATAATGCACATAGTAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTACTATGTGCATTATTCTCGAGAATAATGCACATAGTAGGTGC-3'

Other clones with same target sequence:

(none)