Construct: shRNA TRCN0000159515

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003458.1-9800s1c1
DNA Barcode:
CGAAACTACGTAATGATTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BSN (8927)
Gene Description:
bassoon presynaptic cytomatrix protein
Transcript:
RefSeq NM_003458.1 (NON-CURRENT)
Match location:
Position 9800 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159515)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAACTACGTAATGATTGAT
Hairpin Sequence:
5'-CCGG-CGAAACTACGTAATGATTGAT-CTCGAG-ATCAATCATTACGTAGTTTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAACTACGTAATGATTGATCTCGAGATCAATCATTACGTAGTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAACTACGTAATGATTGATCTCGAGATCAATCATTACGTAGTTTCG-3'

Other clones with same target sequence:

(none)