Construct: shRNA TRCN0000159557

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022459.3-3504s1c1
DNA Barcode:
GCCTGAAACTTTGATATGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPO4 (64328)
Gene Description:
exportin 4
Transcript:
RefSeq NM_022459.3 (NON-CURRENT)
Match location:
Position 3504 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159557)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAAACTTTGATATGTAT
Hairpin Sequence:
5'-CCGG-GCCTGAAACTTTGATATGTAT-CTCGAG-ATACATATCAAAGTTTCAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAAACTTTGATATGTATCTCGAGATACATATCAAAGTTTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAAACTTTGATATGTATCTCGAGATACATATCAAAGTTTCAGGC-3'

Other clones with same target sequence:

(none)