Construct: shRNA TRCN0000159700

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005364.3-656s1c1
DNA Barcode:
GAACCACTTTCCTGATATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGEA8 (4107)
Gene Description:
MAGE family member A8
Transcript:
RefSeq NM_005364.3 (NON-CURRENT)
Match location:
Position 656 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159700)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCACTTTCCTGATATCTT
Hairpin Sequence:
5'-CCGG-GAACCACTTTCCTGATATCTT-CTCGAG-AAGATATCAGGAAAGTGGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCACTTTCCTGATATCTTCTCGAGAAGATATCAGGAAAGTGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCACTTTCCTGATATCTTCTCGAGAAGATATCAGGAAAGTGGTTC-3'

Other clones with same target sequence:

(none)