Construct: shRNA TRCN0000159726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152697.2-689s1c1
DNA Barcode:
GCACTTTAACAATAGGAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC44A5 (204962)
Gene Description:
solute carrier family 44 member 5
Transcript:
RefSeq NM_152697.2 (NON-CURRENT)
Match location:
Position 689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTTAACAATAGGAAGTA
Hairpin Sequence:
5'-CCGG-GCACTTTAACAATAGGAAGTA-CTCGAG-TACTTCCTATTGTTAAAGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTTAACAATAGGAAGTACTCGAGTACTTCCTATTGTTAAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTTAACAATAGGAAGTACTCGAGTACTTCCTATTGTTAAAGTGC-3'

Other clones with same target sequence:

(none)