Construct: shRNA TRCN0000159746

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020675.3-303s1c1
DNA Barcode:
GTTGAGATGTTTCTGGAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPC25 (57405)
Gene Description:
SPC25 component of NDC80 kinetochore complex
Transcript:
RefSeq NM_020675.3 (NON-CURRENT)
Match location:
Position 303 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159746)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGAGATGTTTCTGGAATAT
Hairpin Sequence:
5'-CCGG-GTTGAGATGTTTCTGGAATAT-CTCGAG-ATATTCCAGAAACATCTCAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGAGATGTTTCTGGAATATCTCGAGATATTCCAGAAACATCTCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGAGATGTTTCTGGAATATCTCGAGATATTCCAGAAACATCTCAAC-3'

Other clones with same target sequence:

(none)