Construct: shRNA TRCN0000159834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001548.1-149s1c1
DNA Barcode:
GACGATGAAATGCCTGATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IFIT1 (3434)
Gene Description:
interferon induced protein with tetratricopeptide repeats 1
Transcript:
RefSeq NM_001548.1 (NON-CURRENT)
Match location:
Position 149 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGATGAAATGCCTGATTTA
Hairpin Sequence:
5'-CCGG-GACGATGAAATGCCTGATTTA-CTCGAG-TAAATCAGGCATTTCATCGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGATGAAATGCCTGATTTACTCGAGTAAATCAGGCATTTCATCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGATGAAATGCCTGATTTACTCGAGTAAATCAGGCATTTCATCGTC-3'

Other clones with same target sequence:

(none)