Construct: shRNA TRCN0000159909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004094.3-253s1c1
DNA Barcode:
CGTATCCGTTCTATCAACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF2S1 (1965)
Gene Description:
eukaryotic translation initiation factor 2 subunit alpha
Transcript:
RefSeq NM_004094.3 (NON-CURRENT)
Match location:
Position 253 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000159909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTATCCGTTCTATCAACAAA
Hairpin Sequence:
5'-CCGG-CGTATCCGTTCTATCAACAAA-CTCGAG-TTTGTTGATAGAACGGATACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTATCCGTTCTATCAACAAACTCGAGTTTGTTGATAGAACGGATACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTATCCGTTCTATCAACAAACTCGAGTTTGTTGATAGAACGGATACG-3'

Other clones with same target sequence:

  1. TRCN0000344515