Construct: shRNA TRCN0000160028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032137.2-1491s1c1
DNA Barcode:
CAAGTTTCATTACACCTTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C3orf20 (84077)
Gene Description:
chromosome 3 open reading frame 20
Transcript:
RefSeq NM_032137.2 (NON-CURRENT)
Match location:
Position 1491 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTTTCATTACACCTTCTA
Hairpin Sequence:
5'-CCGG-CAAGTTTCATTACACCTTCTA-CTCGAG-TAGAAGGTGTAATGAAACTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTTTCATTACACCTTCTACTCGAGTAGAAGGTGTAATGAAACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTTTCATTACACCTTCTACTCGAGTAGAAGGTGTAATGAAACTTG-3'

Other clones with same target sequence:

(none)