Construct: shRNA TRCN0000160110

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174938.3-2144s1c1
DNA Barcode:
CCATAATTCACTGCACTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FRMD3 (257019)
Gene Description:
FERM domain containing 3
Transcript:
RefSeq NM_174938.3 (NON-CURRENT)
Match location:
Position 2144 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160110)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAATTCACTGCACTTAAA
Hairpin Sequence:
5'-CCGG-CCATAATTCACTGCACTTAAA-CTCGAG-TTTAAGTGCAGTGAATTATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAATTCACTGCACTTAAACTCGAGTTTAAGTGCAGTGAATTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAATTCACTGCACTTAAACTCGAGTTTAAGTGCAGTGAATTATGG-3'

Other clones with same target sequence:

(none)