Construct: shRNA TRCN0000160170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144707.1-3289s1c1
DNA Barcode:
CCTCTATTCAAAGGCAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PROM2 (150696)
Gene Description:
prominin 2
Transcript:
RefSeq NM_144707.1 (NON-CURRENT)
Match location:
Position 3289 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTATTCAAAGGCAGAAAT
Hairpin Sequence:
5'-CCGG-CCTCTATTCAAAGGCAGAAAT-CTCGAG-ATTTCTGCCTTTGAATAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTATTCAAAGGCAGAAATCTCGAGATTTCTGCCTTTGAATAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTATTCAAAGGCAGAAATCTCGAGATTTCTGCCTTTGAATAGAGG-3'

Other clones with same target sequence:

(none)