Construct: shRNA TRCN0000160189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016430.2-529s1c1
DNA Barcode:
CAATCACAGATAACTATGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIGP (51227)
Gene Description:
phosphatidylinositol glycan anchor biosynthesis class P
Transcript:
RefSeq NM_016430.2 (NON-CURRENT)
Match location:
Position 529 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATCACAGATAACTATGCAA
Hairpin Sequence:
5'-CCGG-CAATCACAGATAACTATGCAA-CTCGAG-TTGCATAGTTATCTGTGATTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATCACAGATAACTATGCAACTCGAGTTGCATAGTTATCTGTGATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATCACAGATAACTATGCAACTCGAGTTGCATAGTTATCTGTGATTG-3'

Other clones with same target sequence:

  1. TRCN0000276417