Construct: shRNA TRCN0000160315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198402.2-852s1c1
DNA Barcode:
CCAGTTATACTTCCACATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HACD2 (201562)
Gene Description:
3-hydroxyacyl-CoA dehydratase 2
Transcript:
RefSeq NM_198402.2 (NON-CURRENT)
Match location:
Position 852 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTATACTTCCACATGAT
Hairpin Sequence:
5'-CCGG-CCAGTTATACTTCCACATGAT-CTCGAG-ATCATGTGGAAGTATAACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTATACTTCCACATGATCTCGAGATCATGTGGAAGTATAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTATACTTCCACATGATCTCGAGATCATGTGGAAGTATAACTGG-3'

Other clones with same target sequence:

(none)