Construct: shRNA TRCN0000160367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005665.2-304s1c1
DNA Barcode:
CGGATAGTAAGTCTTTAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EVI5 (7813)
Gene Description:
ecotropic viral integration site 5
Transcript:
RefSeq NM_005665.2 (NON-CURRENT)
Match location:
Position 304 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATAGTAAGTCTTTAAGAT
Hairpin Sequence:
5'-CCGG-CGGATAGTAAGTCTTTAAGAT-CTCGAG-ATCTTAAAGACTTACTATCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATAGTAAGTCTTTAAGATCTCGAGATCTTAAAGACTTACTATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATAGTAAGTCTTTAAGATCTCGAGATCTTAAAGACTTACTATCCG-3'

Other clones with same target sequence:

(none)