Construct: shRNA TRCN0000160384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021807.2-2933s1c1
DNA Barcode:
CAAGAAGATAACTACCGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXOC4 (60412)
Gene Description:
exocyst complex component 4
Transcript:
RefSeq NM_021807.2 (NON-CURRENT)
Match location:
Position 2933 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAAGATAACTACCGTTTA
Hairpin Sequence:
5'-CCGG-CAAGAAGATAACTACCGTTTA-CTCGAG-TAAACGGTAGTTATCTTCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAAGATAACTACCGTTTACTCGAGTAAACGGTAGTTATCTTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAAGATAACTACCGTTTACTCGAGTAAACGGTAGTTATCTTCTTG-3'

Other clones with same target sequence:

(none)