Construct: shRNA TRCN0000160467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015137.1-2769s1c1
DNA Barcode:
CCACTGTTAAGTAGTATGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFR3A (23167)
Gene Description:
EFR3 homolog A
Transcript:
RefSeq NM_015137.1 (NON-CURRENT)
Match location:
Position 2769 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGTTAAGTAGTATGTTT
Hairpin Sequence:
5'-CCGG-CCACTGTTAAGTAGTATGTTT-CTCGAG-AAACATACTACTTAACAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGTTAAGTAGTATGTTTCTCGAGAAACATACTACTTAACAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGTTAAGTAGTATGTTTCTCGAGAAACATACTACTTAACAGTGG-3'

Other clones with same target sequence:

(none)