Construct: shRNA TRCN0000160578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152994.2-1563s1c1
DNA Barcode:
CAGAAGCTGATAACAACTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1R21 (129285)
Gene Description:
protein phosphatase 1 regulatory subunit 21
Transcript:
RefSeq NM_152994.2 (NON-CURRENT)
Match location:
Position 1563 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAGCTGATAACAACTAAT
Hairpin Sequence:
5'-CCGG-CAGAAGCTGATAACAACTAAT-CTCGAG-ATTAGTTGTTATCAGCTTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAGCTGATAACAACTAATCTCGAGATTAGTTGTTATCAGCTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAGCTGATAACAACTAATCTCGAGATTAGTTGTTATCAGCTTCTG-3'

Other clones with same target sequence:

(none)