Construct: shRNA TRCN0000160593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004600.3-1320s1c1
DNA Barcode:
CGTTTCTTACTAGCTGTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RO60 (6738)
Gene Description:
Ro60, Y RNA binding protein
Transcript:
RefSeq NM_004600.3 (NON-CURRENT)
Match location:
Position 1320 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTTCTTACTAGCTGTTGAT
Hairpin Sequence:
5'-CCGG-CGTTTCTTACTAGCTGTTGAT-CTCGAG-ATCAACAGCTAGTAAGAAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTTCTTACTAGCTGTTGATCTCGAGATCAACAGCTAGTAAGAAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTTCTTACTAGCTGTTGATCTCGAGATCAACAGCTAGTAAGAAACG-3'

Other clones with same target sequence:

(none)