Construct: shRNA TRCN0000160623

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198795.1-4252s1c1
DNA Barcode:
CCTTTGCTTTCATGTTTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TDRD1 (56165)
Gene Description:
tudor domain containing 1
Transcript:
RefSeq NM_198795.1 (CURRENT)
Match location:
Position 4252 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160623)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGCTTTCATGTTTGTTA
Hairpin Sequence:
5'-CCGG-CCTTTGCTTTCATGTTTGTTA-CTCGAG-TAACAAACATGAAAGCAAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGCTTTCATGTTTGTTACTCGAGTAACAAACATGAAAGCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGCTTTCATGTTTGTTACTCGAGTAACAAACATGAAAGCAAAGG-3'

Other clones with same target sequence:

(none)