Construct: shRNA TRCN0000160649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194294.1-325s1c1
DNA Barcode:
CTTTGGAAAGCTATCACATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IDO2 (169355)
Gene Description:
indoleamine 2,3-dioxygenase 2
Transcript:
RefSeq NM_194294.1 (NON-CURRENT)
Match location:
Position 325 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGGAAAGCTATCACATAT
Hairpin Sequence:
5'-CCGG-CTTTGGAAAGCTATCACATAT-CTCGAG-ATATGTGATAGCTTTCCAAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGGAAAGCTATCACATATCTCGAGATATGTGATAGCTTTCCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGGAAAGCTATCACATATCTCGAGATATGTGATAGCTTTCCAAAG-3'

Other clones with same target sequence:

(none)