Construct: shRNA TRCN0000160893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153238.1-1624s1c1
DNA Barcode:
GAAGGAAGAAATGGAGATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LINC00311 (197196)
Gene Description:
long intergenic non-protein coding RNA 311
Transcript:
RefSeq NM_153238.1 (NON-CURRENT)
Match location:
Position 1624 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGAAGAAATGGAGATCAT
Hairpin Sequence:
5'-CCGG-GAAGGAAGAAATGGAGATCAT-CTCGAG-ATGATCTCCATTTCTTCCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGAAGAAATGGAGATCATCTCGAGATGATCTCCATTTCTTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGAAGAAATGGAGATCATCTCGAGATGATCTCCATTTCTTCCTTC-3'

Other clones with same target sequence:

(none)