Construct: shRNA TRCN0000160953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032314.2-269s1c1
DNA Barcode:
GTCTTGGTATCCATCGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COQ5 (84274)
Gene Description:
coenzyme Q5, methyltransferase
Transcript:
RefSeq NM_032314.2 (NON-CURRENT)
Match location:
Position 269 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTGGTATCCATCGTGTTT
Hairpin Sequence:
5'-CCGG-GTCTTGGTATCCATCGTGTTT-CTCGAG-AAACACGATGGATACCAAGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTGGTATCCATCGTGTTTCTCGAGAAACACGATGGATACCAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTGGTATCCATCGTGTTTCTCGAGAAACACGATGGATACCAAGAC-3'

Other clones with same target sequence:

(none)