Construct: shRNA TRCN0000160982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014877.2-6091s1c1
DNA Barcode:
GATGCACAGTTCCCTTTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HELZ (9931)
Gene Description:
helicase with zinc finger
Transcript:
RefSeq NM_014877.2 (NON-CURRENT)
Match location:
Position 6091 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCACAGTTCCCTTTGTTT
Hairpin Sequence:
5'-CCGG-GATGCACAGTTCCCTTTGTTT-CTCGAG-AAACAAAGGGAACTGTGCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCACAGTTCCCTTTGTTTCTCGAGAAACAAAGGGAACTGTGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCACAGTTCCCTTTGTTTCTCGAGAAACAAAGGGAACTGTGCATC-3'

Other clones with same target sequence:

(none)