Construct: shRNA TRCN0000161196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199044.2-332s1c1
DNA Barcode:
GCACTGGTCAATAACTTTGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NSUN4 (387338)
Gene Description:
NOP2/Sun RNA methyltransferase 4
Transcript:
RefSeq NM_199044.2 (NON-CURRENT)
Match location:
Position 332 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000161196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGGTCAATAACTTTGCT
Hairpin Sequence:
5'-CCGG-GCACTGGTCAATAACTTTGCT-CTCGAG-AGCAAAGTTATTGACCAGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGGTCAATAACTTTGCTCTCGAGAGCAAAGTTATTGACCAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGGTCAATAACTTTGCTCTCGAGAGCAAAGTTATTGACCAGTGC-3'

Other clones with same target sequence:

(none)