Construct: shRNA TRCN0000161229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178231.1-590s1c1
DNA Barcode:
GAAATACCAGCTAAGACTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ICA1L (130026)
Gene Description:
islet cell autoantigen 1 like
Transcript:
RefSeq NM_178231.1 (NON-CURRENT)
Match location:
Position 590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000161229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATACCAGCTAAGACTCAA
Hairpin Sequence:
5'-CCGG-GAAATACCAGCTAAGACTCAA-CTCGAG-TTGAGTCTTAGCTGGTATTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATACCAGCTAAGACTCAACTCGAGTTGAGTCTTAGCTGGTATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATACCAGCTAAGACTCAACTCGAGTTGAGTCTTAGCTGGTATTTC-3'

Other clones with same target sequence:

(none)