Construct: shRNA TRCN0000161303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002470.1-3267s1c1
DNA Barcode:
GCTTGCTCAAGAGTCCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYH3 (4621)
Gene Description:
myosin heavy chain 3
Transcript:
RefSeq NM_002470.1 (NON-CURRENT)
Match location:
Position 3267 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000161303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGCTCAAGAGTCCATATT
Hairpin Sequence:
5'-CCGG-GCTTGCTCAAGAGTCCATATT-CTCGAG-AATATGGACTCTTGAGCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGCTCAAGAGTCCATATTCTCGAGAATATGGACTCTTGAGCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGCTCAAGAGTCCATATTCTCGAGAATATGGACTCTTGAGCAAGC-3'

Other clones with same target sequence:

(none)